Trizma® hydrochloride
SIGMA/T5941 - BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
EC Number: 214-684-5
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5941-100G
This image depicts SKU: T5941-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5941-500G
This image depicts SKU: T5941-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T5941-1KG
This image depicts SKU: T5941-1KG
| absorption | ≤0.05 at 290 at 40% |
| application(s) | diagnostic assay manufacturing general analytical life science and biopharma sample preparation |
| assay | ≥99.0% (titration) |
| color | white |
| density | 1.39 g/cm3 at 20—25 °C |
| 1.40 g/cm3 at 20—25 °C | |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected |
| endotoxin and total aerobic microbial count, tested | |
| ≤0.5% water (Karl Fischer) | |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| mp | 150-152 °C |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | Cl.NC(CO)(CO)CO |
| solubility | H2O: 667 mg/mL |
| suitability | suitable for electrophoresis |
| suitable for immunohistochemistry | |
| suitable for molecular biology | |
| technique(s) | cell culture | mammalian: suitable |
| DNA purification: suitable | |
| electrophoresis: suitable | |
| protein extraction: suitable | |
| useful pH range | 7.0-9.0 |
| Application: | Tris-HCl hasbenn used • as a washing and rinsing buffer in an immunohistochemical analysis(1) • as a component of reducing sample buffer, lysis buffer and elution buffer, in a biochemical research involving gel electrophoresis and BioLayer interferometry measurement(2) |
| Features and Benefits: | • Suitable for Molecular Biology and Cell Culture • Can be used as a Buffer component, for Electrophoresis and Protein separation • Tested for Endotoxins and Total Aerobic Microbial Count • Free from DNase, NICKase, RNase, and Protease • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C) • Highly soluble in water |
| General description: | Tris HCl, also known as Trizma® hydrochloride, is a widely used acidic buffer for preparing buffers within the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, Tris HCl offers a pH range of 7.0 to 9.0, matching the typical pH of living organisms. As a result, Tris HCl is an essential component of buffer solutions used in various biological, cell culture, biochemistry, and molecular biology applications. It is important to note that neither Tris base nor Tris hydrochloride on their own can provide sufficient buffering capacity. Therefore, they are typically combined to create a buffer within the pH range of 7 to 9, ensuring effective pH control. Tris buffers are indispensable in techniques like protein electrophoresis and western blotting. In many western blot protocols, a low ionic strength Tris buffer is employed for protein transfer. The transfer duration depends on the blotting apparatus and the size range of peptides under investigation. Tris-based buffers are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Furthermore, Tris salts find extensive use in DNA agarose electrophoresis, particularly in the preparation of buffers like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA). These buffers play a pivotal role in various applications, including biochemistry, protein purification, and electrophoresis. Tris HCl is a versatile and essential for maintaining the pH of biological systems and ensuring the optimal performance of biochemical, cell culture, and molecular biology techniques. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | T5941-1KT: Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (titration) |
| mp | 150-152 °C |
| Density | 1.39 g/cm3 at 20—25 °C; 1.40 g/cm3 at 20—25 °C |
| UNSPSC | 12161700 |