Trizma® hydrochloride solution
SIGMA/T2819 - pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture
Synonym: Tris hydrochloride solution
Product Type: Chemical
| absorption | ≤0.05 at 290 at 40% |
| concentration | 1 M |
| form | solution |
| grade | BioPerformance Certified |
| Molecular Biology | |
| impurities | bioburden, tested |
| DNase, RNase, Protease, none detected | |
| endotoxin, tested | |
| ≤5 ppm Heavy metals (as Pb) | |
| pH | 9.0 |
| Quality Level | 200  |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7.0-9.0 |
| Application: | Trizma® hydrochloride solution has been used: • to neutralize IgG bound to protein A/G column • as a component in BigDye buffer for sequencing reaction • to block slides |
| General description: | A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units. |
| General description: | Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |