Trizma^® base

SIGMA/T1503 - Primary Standard and Buffer, ≥99.9% (titration), crystalline

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol

CAS Number: 77-86-1
Empirical Formula (Hill Notation): C₄H₁₁NO₃
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH₂C(CH₂OH)₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T1503-25G	25 g	$26.70 1/EA	Add To Favorites
45-T1503-100G	100 g	$41.10 1/EA	Add To Favorites
45-T1503-250G	250 g	$75.10 1/EA	Add To Favorites
45-T1503-500G	500 g	$113.00 1/EA	Add To Favorites
45-T1503-1KG	1 kg	$186.00 1/EA	Add To Favorites
45-T1503-5KG	5 kg	$789.00 1/EA	Add To Favorites
45-T1503-10KG	10 kg	$1300.00 1/EA	Add To Favorites
45-T1503-25KG	25 kg	$2760.00 1/EA	Add To Favorites
45-T1503-25KG-DP	25 kg	No Price
45-T1503-50KG	50 kg	$4260.00 1/EA	Add To Favorites

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This image depicts SKU: T1503-100G

Properties
Descriptions
Safety Info.
Basic Data

absorption	≤0.05 at 290 nm at 40%
application(s)	cell analysis diagnostic assay manufacturing life science and biopharma
assay	≥99.9% (titration)
bp	219-220 °C/10 mmHg (lit.)
color	white
description	aminopeptidase substrate
form	crystalline
InChI	1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key	LENZDBCJOHFCAS-UHFFFAOYSA-N
mp	167-172 °C (lit.)
pH	10.5-12
pKa (25 °C)	8.1
Quality Level	300
SMILES string	NC(CO)(CO)CO
solubility	ethylene glycol: soluble 79.1 mg/mL at 25 °C
	methanol: soluble 26 mg/mL at 25 °C
	water: soluble (678 g/l at 20 °C)
storage condition	dry at room temperature
suitability	suitable for electrophoresis
	suitable for Western blot
technique(s)	ELISA: suitable
	protein extraction: suitable
useful pH range	7-9

Application:	Trizma^® base has been used: • as a component of H buffer (cell dissociation buffer) • for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique • as an assay buffer for reconstitution of extracted and dried protein samples • to prepare Tris-HCl buffer that is used to stabilize proteins • as a buffer to extract carotenoid from tubers • as a component of sample buffer during protein extraction prior to western blotting • as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) • to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay • as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
Features and Benefits:	• Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C) • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Can be used in Cell Biology, and Biochemical research
General description:	Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma^® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes. Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.
Legal Information:	DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Other Notes:	The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 250, 500 g in poly bottle
Packaging:	5, 10, 25, 50 kg in poly drum
Packaging:	The DRYPOUR^® packaging system for high-quality hygroscopic salts DRYPOUR^® packaging: • The current DRYPOUR^® packaging configuration is in a 50L nestable drum • 25KG-DP are the standard prepack offering • Smaller volumes can be put in the same drum for variable configurations The benefits of DRYPOUR^®: Improves operational efficiency by reducing material preparation time and increasing operator safety Minimizes caking of hygroscopic salts Offers triple protection: against moisture inside and outside, and against contamination How does the DRYPOUR^® packaging system Improve your operational efficiency? This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek^® liner Desiccant bags are firmly integrated into the polyethylene liner • Triple protection delivers two invaluable results: Dramatically reduced caking No contamination risk from the desiccant

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.9% (titration)
bp	219-220 °C/10 mmHg (lit.)
mp	167-172 °C (lit.)
UNSPSC	12352104

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