holo-Transferrin human
SIGMA/T0665 - powder, BioReagent, suitable for cell culture, ≥97%
Synonym: Siderophilin, iron-saturated
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL Number: MFCD00131337
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T0665-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T0665-500MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T0665-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T0665-1G
| assay | ≥97% |
| biological source | human plasma |
| description | Iron-saturated |
| form | powder |
| impurities | endotoxin, tested |
| HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods) | |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | H2O: 50 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02787  |
| Analysis Note: | Iron content by ICP reported on a lot-specific basis. |
| Analysis Note: | Purity by agarose gel electrophoresis. |
| Application: | holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy. |
| Application: | Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media. |
| Biochem/physiol Actions: | Provides iron to cells in a physiologically stable and safe form. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 50, 100, 500 mg in poly bottle |
| Preparation Note: | Heat treated at 60 °C (minimum) for a minimum of 10 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥97% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |