LAG-3 (human): FC (human)
SIGMA/SRP8040 - recombinant, expressed in CHO cells, >99% (SDS-PAGE)
Synonym: FDC Protein; Lymphocyte activation gene-3; PC cell-derived growth factor
Product Type: Chemical
Histogram LAG-3 (Human): FC (Human): Cat. No. SRP8040: LAG-3 (Human): FC (Human)-induced dendritic cells (DC) maturation. Method: I mMature DCs derived from Human monocytes, re-suspended at 1 × 106 cells/mL in complete culture medium, are incubated with LAG-3 (Human): FC (Human) (10 βg/mL), LPS (positive control, 5 βg/mL) or Human IgG1 (isotype-matched negative control, 10 βg/mL) for 48 hours. DC maturation induced by LAG-3 (Human): FC (Human) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
| assay | >99% (SDS-PAGE) |
| biological source | human |
| color | clear |
| concentration | ≥0.2 mg/mL |
| form | liquid |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | ~80 kDa by SDS-PAGE |
| packaging | pkg of 50 μg |
| recombinant | expressed in CHO cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P18627   |
| Biochem/physiol Actions: | LAG3 (lymphocyte activation gene 3) is a negative regulator of T-cell proliferation where it suppresses T-cell receptor (TCR)-mediated calcium fluxes and modulates the memory T-cell pool size. It is a CD4 (cluster of differentiation) homolog that functions as a ligand for MHC (major histocompatibility complex) II. Membrane expression of LAG3 modulates the suppressive function of Tregs (T-regulatory) both in vivo and in vitro. HIV-1 (human immunodeficiency virus) infection results in significant increase in LAG3 peripheral blood and lymph node expression, which is exhibited on both CD4+ and CD8+ T cells, and this is linked with disease progression. In melanoma, this protein is responsible for TLR (Toll-like receptor)-independent activation of plasmacytoid dendritic cells (pDCs) at tumor sites, which is partially implicated in driving an immune-suppressive environment. |
| General description: | LAG3 (lymphocyte activation gene 3) is an inhibitory CD4 (cluster of differentiation)-related molecule, and is expressed by activated CD4+ and CD8+ T cells. |
| Other Notes: | The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1. |
| Physical form: | Solution in PBS. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >99% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |