TRAIL, Soluble human
SIGMA/SRP8024 - recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)
Synonym: Apo-2L; CD253; CD339; TRAIL
Product Type: Chemical
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
| assay | >98% (SDS-PAGE) |
| biological source | human |
| color | clear |
| concentration | ≥0.2 mg/mL |
| form | liquid |
| impurities | <0.01 EU/μg endotoxin, tested |
| mol wt | ~26 kDa |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| tag | His tagged |
| technique(s) | activity assay: suitable |
| cell culture | mammalian: suitable | |
| transfection: suitable | |
| UniProt accession no. | P50591   |
| Application: | TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay. |
| Biochem/physiol Actions: | Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B). |
| General description: | TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31. |
| Other Notes: | The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge. |
| Physical form: | Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >98% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |