Cathepsin D human
SIGMA/SRP6415 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: CLN10; CPSD; CTSD
Product Type: Chemical
Immunoblotting Cathepsin D Cat. No. SRP6415, SDS PAGE of human recombinant Cathepsin D.
| application(s) | life science and biopharma |
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | calculated mol wt 43.6 kDa |
| observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage condition | dry at room temperature |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| tag | 6-His tagged (C-terminus) |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P07339   |
| Application: | Cathepsin D has been used in the protease digestion of haemoglobin. |
| Biochem/physiol Actions: | Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered an important biomarker for type 2 diabetes-dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.Cathepsin D is involved in various physiological processes like apoptosis, autophagy, and protein degradation. Cathepsin D plays an important role in the pathogenesis of Alzheimer’s disease, neuronal ceroid lipofuscinosis, and breast cancer. |
| General description: | Research area: Cell signalling. Cathepsin D belongs to the peptidase A1 family, an estrogenic-induced lysosomal protease. Cathepsin D can be cleaved into the following 2 chains: N-terminal light chain and C-terminal heavy chain, which is expressed in the aorta extracellular space (at the protein level). ). It is found in most mammalian cells and is located in thelysosomes. |
| General description: | This gene is mapped to human chromosome 11p15.5. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |