MISSION® TRC2 pLKO.5-puro TurboGFP™ shRNA Control Plasmid DNA
SIGMA/SHC204 - shRNA sequence targeting tGFP
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
TRC2 shRNA to TurboGFP Control Vector
| concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line | MISSION® |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens. |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | The MISSION® TRC2 Control Vector pLKO-puro TurboGFP™ shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP™. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP™ shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION® TurboGFP™ positive control vector in cell lines expressing TurboGFP™. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP™ shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | TurboGFP is a trademark of Evrogen Co. |
| Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |