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MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles

SIGMA/SHC016V - Targets no known genes from any species

Synonym: MISSION®; MISSION® Control Transduction Particles; negative control; negative shRNA control; non-target control; non-target shRNA; non-target shRNA control; shRNA control

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SHC016VN-1EA 1 ea
 No Price  
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

concentration ≥1x106 VP/ml (via p24 assay)
product line MISSION®
Quality Level 100 
shipped in dry ice
storage temp. −70°C
Analysis Note: To see more application data, protocols, vector maps visit sigma.com/shrna .
Application: MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.
General description: The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
General description: When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna .
Legal Information: MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR UN 3245 9
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −70°C
UNSPSC 41106609

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