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MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles

SIGMA/SHC003V - Green fluorescent protein marker to monitor transduction efficiency

Synonym: MISSION®; MISSION® TurboGFP™ Control Transduction Particles

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SHC003VN 1 each
 No Price  
TRC1 TurboGFP Control Vector

 

concentration ≥1x106 VP/ml (via p24 assay)
product line MISSION®
Quality Level 100 
shipped in dry ice
storage temp. −70°C
technique(s) capture ELISA: 106 TU/mL using p24
Application: MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.
General description: This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
General description: When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna .
Legal Information: MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: TurboGFP is a trademark of Evrogen Co.
RIDADR UN 3245 9
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −70°C
UNSPSC 41106609

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