Anti-phospho-Werner Syndrome Helicase (pSer1141) antibody produced in rabbit
SIGMA/SAB4504327 - affinity isolated antibody
Synonym: RECQ3; RECQL2; WRN
Product Type: Chemical
Western Blotting Anti-phoshpho-Werner Syndrome Helicase (pSer1141): Cat. No. SAB4504327: Western blot analysis of extracts from K562 cells treated with 25 μM Etoposide for 24 hours.
ELISA Anti-phoshpho-Werner Syndrome Helicase (pSer1141): Cat. No. SAB4504327: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 162 kDa |
| NCBI accession no. | Q14191   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer1141) |
| technique(s) | ELISA: 1:5000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q14191 |
| Biochem/physiol Actions: | Werner syndrome, RecQ helicase-like (WRN) can interact with DNA polymerase δ (POLD1) and replication protein A (RPA) that are essential for the replication and repair of DNA. It also interacts with Ku protein which participates in double-strand DNA break repair. Mutation of the WRN gene results in Werner syndrome. WRN is a mortal target for cancer cells with microsatellite instability (MSI). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Werner syndrome, RecQ helicase-like (WRN) protein belongs to the RecQ family of DNA helicases. It has an adenosine triphosphate (ATP) binding site, one DExH helicase box, one exonuclease domain, and a nuclear localization signal. It is predominantly located in the nucleus. The WRN gene is located on human chromosome 8p12. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Werner Syndrome Helicase around the phosphorylation site of Ser1141. Immunogen Range: 1107-1156 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |