Anti-ARHGEF10 antibody produced in rabbit
SIGMA/SAB4503682 - affinity isolated antibody
Synonym: ARHGEF10; Rho guanine nucleotide exchange factor 10
Product Type: Chemical
Immunohistochemistry Anti-ARHGEF10: Cat. No. SAB4503682: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-ARHGEF10 antibody. Cells on the left were treated with the Anti-ARHGEF10 antibody. Cells on the right (negative control) were treated with both Anti-ARHGEF10 antibody and the synthesized immunogen peptide.
Western Blotting Anti-ARHGEF10: Cat. No. SAB4503682: Immunoblotting analysis of extracts from COS7 cells, using Anti-ARHGEF10 antibody. The lane on the left was treated with the Anti-ARHGEF10 antibody. The lane on the right (negative control) was treated with both Anti-ARHGEF10 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 151 kDa |
| NCBI accession no. | O15013   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O15013 |
| Biochem/physiol Actions: | ARHGEF10 (Rho guanine nucleotide exchange factor 10) works as a guanine nucleotide exchange factor for RhoA, RhoB and RhoC. It is involved in atherosclerotic cerebral infarction and mutation in ARHGEF10 is associated with increased risk of atherothrombotic stroke. Mutation in ARFGEF10 is also linked with slowed nerve conduction velocities and thin myelination of peripheral nerves. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-ARHGEF10 Antibody detects endogenous levels of total ARHGEF10 protein. The gene ARHGEF10 (Rho guanine nucleotide exchange factor 10) is mapped to human chromosome 8p23. It belongs to the Rho-specific guanine nucleotide exchange factor family. The protein contains tandem Dbl homology (DH) domain, pleckstrin homology (PH) domain and putative transmembrane domains. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ARHGEF10. Immunogen Range: 339-388 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |