Anti-4E-BP1 antibody produced in rabbit
SIGMA/SAB4500736 - affinity isolated antibody
Synonym: 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6
MDL Number: MFCD04040379
Product Type: Chemical
Immunohistochemistry Anti-4E-BP1: Cat. No. SAB4500736: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-4E-BP1 antibody. Cells on the left were treated with the Anti-4E-BP1 antibody. Cells on the right (negative control) were treated with both Anti-4E-BP1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-4E-BP1: Cat. No. SAB4500736: Immunoblotting analysis of extracts from Jurkat cells, treated with Insulin 0.01U/ml 15′, using Anti-4E-BP1 antibody. The lane on the left was treated with the Anti-4E-BP1 antibody. The lane on the right (negative control) was treated with both Anti-4E-BP1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 12 kDa |
| NCBI accession no. | Q13541   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13541 |
| Application: | Anti-4E-BP1 antibody has been used in western blotting. |
| Biochem/physiol Actions: | E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It also serves as a substrate for mTORC1 (mammalian target of rapamycin complex 1). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-4E-BP1 Antibody detects endogenous levels of total 4E-BP1 protein. |
| General description: | The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is located on human chromosome 8p11. 4E-BP1 is a multifactor regulated multifunctional protein. It belongs to the translation repressor protein family. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human 4E-BP1. Immunogen Range: 30-79 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |