Anti-PP2A subunit B isoform PR55-α antibody, Mouse monoclonal
SIGMA/SAB4200241 - clone 2G9, purified from hybridoma cell culture
Synonym: Anti-B55α; Anti-PPP2R2A; Anti-PR52A; Anti-PR55A; Anti-Protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform; Anti-Protein phosphatase 2, regulatory subunit B, alpha, B55A; Anti-
Product Type: Chemical
Immunoblotting Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2A subunit B isoform PR55-alpha Clone: 2G9 (Cat. No. SAB4200241). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2A subunit B isoform PR55-alpha Clone: 2G9 (Cat. No. SAB4200241). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
Immunoblotting Whole extract of NRK cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2A subunit B isoform PR55-alpha Clone: 2G9 (Cat. No. SAB4200241). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G9, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 50-55 kDa |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-2 μg/mL using whole extracts of mouse 3T3, rat NRK or human HeLa cells | |
| UniProt accession no. | P63151   |
| Application: | Monoclonal Anti-PP2A subunit B isoform PR55-α antibody produced in mouse has been used in: • immunoblotting • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ. |
| Biochem/physiol Actions: | PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells . Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PP2A subunit B isoform PR55- (mouse IgG1 isotype) is derived from the hybridoma 2G9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of rat PP2A subunit B isoform PR55-. PP2A subunit B isoform PR55- (PPP2R2A) belongs to the phosphatase (PP)2A regulatory subunit B family. Protein phosphatase 2A is one of the major Ser/Thr phosphatases. PPP2R2A encodes an alpha isoform of the regulatory subunit B55 subfamily. This gene is mapped to human chromosome 8p21.2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |