Monoclonal Anti-Lamin A/C antibody produced in mouse
SIGMA/SAB4200236 - clone 4C11, purified from hybridoma cell culture
Synonym: Anti-CDCD1; Anti-CDDC; Anti-CMD1A; Anti-CMT2B1; Anti-EMD2; Anti-FPL; Anti-FPLD; Anti-HGPS; Anti-IDC; Anti-LDP1; Anti-LFP; Anti-LGMD1B; Anti-LMN1; Anti-LMNA; Anti-LMNC; Anti-LMNL1; Anti-PRO1; Anti-renal carcinoma antigen NY-REN-32
MDL Number: MFCD01633588
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.2 μg/mL Monoclonal Anti-Lamin A/C Clone: 4C11 (Cat. No. SAB4200236). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. G361; 3. HepG2; 4. NIH-3T3; 5. MDCK; 6. BMGE
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by 0.5% Triton X-100. Fixed cells were stained with 0.5 μg/mL Monoclonal Anti-Lamin A/C Clone: 4C11 (Cat. No. SAB4200236). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoprecipitation Monoclonal Anti-Lamin A/C Clone: 4C11 (Cat. No. SAB4200236) was used to immunoprecipitate Lamin A/C from HeLa whole cell extract. Detection antibody: Monoclonal Anti Lamin A/C Clone: 4C11 (Cat. No. SAB4200236) Lanes 1. Positive control: HeLa whole cell extract 2. 10 μg/mLg antibody 3. 5 μg/mLg antibody 4. 2.5 μg/mLg antibody 5. Negative control: 5 μg/mLg non-relevant antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C11, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~72/63 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, canine, human, monkey, hamster, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 0.1-0.2 μg/mL using whole extracts of human HeLa cells | |
| UniProt accession no. | P02545  |
| Application: | Mouse monoclonal clone 4C11 anti-Lamin A/C antibody is used to tag lamins A and/or C for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of lamins A and/or C in nuclear envelope structure and function. This antibody may be used in several immunochemical techniques including immunoblotting (~72/63 kDa), immunoprecipitation and immunofluorescence. |
| Biochem/physiol Actions: | Lamins expressed in somatic cells interact with chromatin, nuclear pore complexes and integral proteins of the inner membrane of the nuclear envelope, such as LAPs 1 and 2 (lamin associated polypeptides), LBR (Lamin B receptor) and emerin. Mutations in Lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome). Lamin A is cleaved into a 47 kDa fragment during apoptosis. Lamin A cleavage seems to be essential for chromatin condensation and nuclear disassembly in apoptosis. |
| Biochem/physiol Actions: | Mouse monoclonal clone 4C11 anti-Lamin A/C antibody recognizes human, rat, mouse, canine, hamster, monkey and bovine Lamin A/C. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Lamin A is a structural protein of the nuclear lamina, a meshwork of intermediate filaments that underlies the inner face of the nuclear envelope. The major components of the nuclear lamina are the lamins that may be classified into two types, A and B. Both A- and B- type lamins are characterized by an a-helical rod domain to enable assembly into filaments, a nuclear localization sequence, and a C-terminal CAAX box isoprenylation sequence for nuclear membrane targeting. A-type lamins, A and C, are produced by alternative splicing resulting in proteins of 664 and 572 amino acids, respectively. The first 566 amino acids of Lamins A and C are identical. Prelamin A, the precursor of Lamin A, has 98 unique amino acids and is farnesylated at its carboxy terminus after synthesis. The last 18 amino acids, which contain the farnesyl group, are removed by an endoproteolytic cleavage, producing the mature Lamin A. Monoclonal Anti-Lamin A/C (mouse IgG2a isotype) is derived from the hybridoma 4C11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with the Ig-fold domain of human Lamin A. |
| Immunogen: | hybridoma 4C11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with the Ig-fold domain of human Lamin A |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |