Anti-TDP-43 (N-terminal region) antibody produced in rabbit
SIGMA/SAB4200006 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ALS10; Anti-TARDBP; Anti-TARDP43; Anti-Tar DNA binding protein 43
Product Type: Chemical
Immunohistochemistry Nuclear localization of TDP-43 in Human kidney. Fixed tissue was stained with 5 μg/mL Anti-TDP-43 (N-terminal region) (Cat. No. SAB4200006) followed by Goat Anti-Rabbit IgG, HRP conjugate (×40 magnification).
Immunohistochemistry Nuclear localization of TDP-43 in Mouse kidney. Fixed tissue was stained with 5 μg/mL Anti-TDP-43 (N-terminal region) (Cat. No. SAB4200006) followed by Goat Anti-Rabbit IgG, HRP conjugate (×40 magnification).
Immunofluorescence Nuclear localization of TDP-43 in HepG2 cells. Fixed cells were stained with 5 μg/mL Anti-TDP-43 (N-terminal region) (Cat. No. SAB4200006) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Tissue and cells extract were separated on SDS-PAGE, blotted with 1 μg/mL Anti-TDP-43 (N-terminal region) (Cat. No. SAB4200006), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat spinal cord; 2. Mouse lung; 3. HEK-293T; 4. K562; 5. HepG2; 6. HeLa; 7. U2OS; 8. U2OS. Antibody + TDP-43 immunizing peptide (human, 75-90)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 5-10 μg/mL using human or mouse kidney |
| indirect immunofluorescence: 5-10 μg/mL using HepG2 cells | |
| western blot: 1.5-3.0 μg/mL using HepG2 cell lysates | |
| UniProt accession no. | Q13148   |
| Application: | Anti-TDP-43 (N-terminal region) antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunohistochemistry • immunuprecipitation |
| Biochem/physiol Actions: | TDP-43 is also involved in mediating the transcription regulation of human immune deficiency virus (HIV). TDP-43 has been identified as the major ubiquinated component of cytoplasmic inclusions in frontotemporal lobe degeneration subtype FTLD-U and amyotrophic lateral sclerosis (ALS). Pathological TDP-43 forms abnormal inclusions in neuronal perikarya and neurites, indicating that redistribution of TDP-43 to the cytoplasm is a pathogenic mechanism. Several pathogenic TDP43 mutations have been identified in familial ALS, causing aberrant cleavage of TDP-43 to C-terminal fragments, and predisposing nuclear TDP-43 to redistribute to the cytoplasm and form pathological aggregates. Abnormal phosphorylation of TDP-43 at Ser409/410 has also been observed in frontotemporal lobar degeneration and amyotrophic lateral sclerosis (FTLD-U and ALS) suggesting a toxic gain of function leading to apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TDP-43 (TAR DNA binding protein, TARDP) belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs) that bind single stranded RNA. TDP-43 is predominantly localized to the nucleus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |