Monoclonal Anti-Ubiquitin antibody produced in mouse
SIGMA/SAB2702287 - clone GT751, affinity isolated antibody
Product Type: Product-on-demand
Western Blotting Ubiquitin antibody detects Ubiquitin protein by western blot analysis. Non-transfected (-) and -transfected (+, including Myc-tag) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody (SAB2702287) diluted by 1:10,000.
Western Blotting Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT751] (SAB2702287) diluted at 1:500.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT751, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000 |
| western blot: 1:500-1:20,000 |
| Application: | Suggested starting dilutions are as follows: WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | The immunogen used to generate this antibody corresponds to Ubiquitin |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |