Monoclonal Anti-GFP antibody produced in mouse
SIGMA/SAB2702197 - clone GT859, affinity isolated antibody
Synonym: Mouse Monoclonal Anti-Green Fluorescent Protein
Product Type: Product-on-demand
Immunofluorescence GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (SAB2702197) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting GFP antibody detects GFP protein by western blot analysis. A. 5 μg 293T whole cell extract B. 5 μg 293T whole cell extract expressing GFP-tagged protein 12% SDS-PAGE GFP antibody (SAB2702197) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (SAB2702197) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (SAB2702197) diluted at 1:1000.
ELISA ELISA detection of GFP using SAB2702197 for capture. Coating Ab: mouse anti-GFP (SAB2702197, 2μg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT859, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1000-10000 |
| Application: | Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IHC-Fr: Assay-dependent dilution, IHC-P: Assay-dependent dilution, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-GFP antibody has been used in gait analysis. |
| Biochem/physiol Actions: | Green fluorescent protein (GFP) is used as a marker for gene expression. It is also used as a tag to study about protein localization in several organisms. GFP can form the intrinsic chromophore without the help of cofactors or enzymatic components. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Green fluorescent protein (GFP) is a fluorescent protein. It is mainly isolated from the luminous organ of the jellyfish Aequorea victoria. |
| Immunogen: | Full length GFP recombinant protein |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |