Anti-NOX1
SIGMA/SAB2108601 - affinity isolated antibody
Synonym: Anti- MOX1; Anti- NOH-1; Anti- NOH1; Anti-GP91-2
Product Type: Product-on-demand
Immunoblotting Cell Type: Jurkat (Cat. No. SAB2108601). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type Jurkat
Western Blotting Western Blot of NOX1 in Human CaCO3 with NOX1 antibody at 1 μg/mL
Western Blotting Western Blot of NOX1 in Human MCF7 Whole Cell with NOX1 antibody at 1 μg/mL
Western Blotting Western Blot of NOX1 in Human Jurkat Whole Cell with NOX1 antibody at 1 μg/mL
| accession no. | NM_007052 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 65 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rabbit, horse, human, rat, dog, bovine, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| UniProt accession no. | Q9Y5S8  |
| Biochem/physiol Actions: | Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox.Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. The NOH-1S currents were reversibly blocked by zinc, a known H+ channel inhibitor. The NOH-1S variant does not contain an electron transport chain and it is thought that H+ conductance is its main physiologic function, whereas NOH-1L may conduct H+ ions as part of its electron transport mechanism. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human NOX1 |
| Other Notes: | Synthetic peptide located within the following region: STIATSHPKSVVGVFLCGPRTLAKS |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |