Anti-SHH
SIGMA/SAB2108581 - affinity isolated antibody
Synonym: Anti- HLP3; Anti- HPE3; Anti- SMMCI; Anti- TPT; Anti-HHG1
Product Type: Product-on-demand
Immunohistochemistry Anti-SHH: Cat. No. SAB2108581: Immunohistochemistry of SHH in Chicken Embryos tissue with SHH antibody at 1μg/mL.
Immunohistochemistry Immunohistochemistry of SHH in Chicken embryos with SHH antibody at 4-8 μg/mL
Immunohistochemistry Immunohistochemistry of SHH in Human glioma with SHH antibody at 4-8 μg/mL
Immunohistochemistry Immunohistochemistry of SHH in Human Urinary Bladder with SHH antibody at 4-8 μg/mL
Immunoblotting Cell Type: HepG2 (Cat. No. SAB2108581). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type HepG2
Western Blotting Western Blot of SHH in Human Stomach Tumor with SHH antibody at 1 μg/mL
Western Blotting Western Blot of SHH in Mouse Brain with SHH antibody at 1 μg/mL
| accession no. | NM_000193 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 28 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | guinea pig, human, horse, mouse, rabbit, rat, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q15465  |
| Biochem/physiol Actions: | SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human SHH |
| Other Notes: | Synthetic peptide located within the following region: RCLLLVLVSSLLVCSGLACGPGRGF |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |