Anti-ATG5 antibody produced in rabbit
SIGMA/SAB2108076 - affinity isolated antibody
Product Type: Product-on-demand
Immunoblotting Cell Type: HepG2 (Cat. No. SAB2108076). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type HepG2
Western Blotting Western Blot of ATG5 in Human Fetal Lung with ATG5 antibody at 1 μg/mL
Western Blotting Western Blot of ATG5 in Human Fetal Liver with ATG5 antibody at 1 μg/mL
Western Blotting Western Blot of ATG5 in Human Fetal Heart with ATG5 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 32kDa |
| NCBI accession no. | NM_004849   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, guinea pig, rat, horse, dog, rabbit, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q9H1Y0 |
| Biochem/physiol Actions: | ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human ATG5 |
| Other Notes: | Synthetic peptide located within the following region: DPEDGEKKNQVMIHGIEPMLETPLQ |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |