Anti-DPH1, (N-terminal) antibody produced in rabbit
SIGMA/SAB2105796 - affinity isolated antibody
Synonym: Anti-DPH2L; Anti-DPH2L1; Anti-FLJ33211; Anti-OVCA1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of DPH1 in Human liver tissue with DPH1 antibody at 4-8 μg/mL
Immunoblotting Anti-DPH1 (N-Terminal): Cat. No. SAB2105796: DPH1 in RPMI-8226 cell lysates with DPH1 antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 49 kDa |
| NCBI accession no. | NM_001383   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, dog, human, rabbit, rat, guinea pig, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9BZG8 |
| Biochem/physiol Actions: | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human DPH1 |
| Other Notes: | Synthetic peptide located within the following region: NQIPPEILKNPQLQAAIRVLPSNYN |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |