ANTI-EPHB3 antibody produced in mouse
SIGMA/SAB1412401 - clone 3F12, purified immunoglobulin, buffered aqueous solution
Synonym: EPHB3; ETK2; HEK2; TYRO6
Product Type: Chemical
Western Blotting EPHB3 monoclonal antibody, clone 3F12 Western Blot analysis of EPHB3 expression in NIH/3T3.
Western Blotting Western Blot analysis of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody, clone 3F12. Lanes Lane 1: EPHB3 transfected lysate (Predicted MW: 110.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of EPHB3 transfected lysate using anti-EPHB3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with EPHB3 monoclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen 36.63 kDa |
| NCBI accession no. | NM_004443   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | , human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P54753 |
| General description: | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinosi |
| Immunogen: | EPHB3 (NP_004434, 899 a.a. ~ 997 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AASLKVIASAQSGMSQPLLDRTVPD |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |