Anti-NCL antibody produced in mouse
SIGMA/SAB1409341 - purified immunoglobulin, buffered aqueous solution
Synonym: C23; FLJ45706
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to NCL on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NCL expression in transfected 293T cell line by NCL polyclonal antibody. Lanes Lane 1: NCL transfected lysate (76.6 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 126.6 kDa |
| NCBI accession no. | NM_005381.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P19338 |
| General description: | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. (provided by RefSeq) |
| Immunogen: | NCL (NP_005372.2, 1 a.a. ~ 710 a.a) full-length human protein. Sequence MVKLAKAGKNQGDPKKMAPPPKEVE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |