Monoclonal Anti-MYCBP, (C-terminal) antibody produced in mouse
SIGMA/SAB1405016 - clone 1F10, purified immunoglobulin, buffered aqueous solution
Synonym: AMY-1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MYCBP on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of MYCBP expression in transfected 293T cell line by MYCBP monoclonal antibody, clone 1F10. Lanes Lane 1: MYCBP transfected lysate (12 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.44 kDa).
ELISA Detection limit for recombinant GST tagged MYCBP is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~33.81 kDa |
| NCBI accession no. | NM_012333   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99417 |
| General description: | The MYCBP gene encodes a protein that binds to the N-terminal region of MYC (MIM 190080) and stimulates the activation of E box-dependent transcription by MYC.[supplied by OMIM |
| Immunogen: | MYCBP (NP_036465.2, 34 a.a. ~ 103 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LYEEPEKPNSALDFLKHHLGAATPE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |