Monoclonal Anti-AGT antibody produced in mouse
SIGMA/SAB1403532 - clone 3C10, purified immunoglobulin, buffered aqueous solution
Synonym: ANHU; FLJ92595; FLJ97926; SERPINA8
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to AGT on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of AGT expression in transfected 293T cell line by AGT monoclonal antibody, clone 3C10. Lanes Lane 1: AGT transfected lysate (Predicted MW: 53.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (79.09 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3κ |
| mol wt | antigen ~79.46 kDa |
| NCBI accession no. | BC011519   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P01019 |
| General description: | The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. (provided by RefSeq) |
| Immunogen: | AGT (AAH11519, 1 a.a. ~ 485 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRKRAPQSEMAPAGVSLRATILCLL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |