Monoclonal Anti-ACO1, (C-terminal) antibody produced in mouse
SIGMA/SAB1403517 - clone 2F6, purified immunoglobulin, buffered aqueous solution
Synonym: ACONS; IREB1; IREBP; IREBP1; IRP1
Product Type: Chemical
Immunoblotting Monoclonal Anti-ACO1: Cat. No. SAB1403517: Antibody concentration: 1 μg/mL. Specific band of ~38.2 kDa using immunogen protein lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~38.21 kDa |
| NCBI accession no. | BC018103   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P21399 |
| General description: | Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5′ UTR of ferritin mRNA, and in the 3′ UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. (provided by RefSeq) |
| Immunogen: | ACO1 (AAH18103, 780 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RDWAAKGPFLLGIKAVLAESYERIH |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |