Monoclonal Anti-MED9 antibody produced in mouse
SIGMA/SAB1403279 - clone 7F1, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ10193; MED25; MGC138234
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MED9 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting QC Western Blot detection against Immunogen (42.8 kDa).
ELISA Detection limit for recombinant GST tagged MED9 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~43.4 kDa |
| NCBI accession no. | NM_018019   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NWA0 |
| General description: | The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. (provided by RefSeq) |
| Immunogen: | MED9 (NP_060489.1, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASAGVAAGRQAEDVLPPTSDQPLP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |