Monoclonal Anti-VCAM1 antibody produced in mouse
SIGMA/SAB1402959 - clone 1H6, purified immunoglobulin, buffered aqueous solution
Synonym: CD106; DKFZp779G2333; INCAM-100; MGC99561
Product Type: Chemical
Western Blotting Western Blot analysis of VCAM1 expression in transfected 293T cell line by VCAM1 monoclonal antibody, clone 1H6. Lanes Lane 1: VCAM1 transfected lysate (Predicted MW: 81.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (106.81 kDa).
ELISA Detection limit for recombinant GST tagged VCAM1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~107.4 kDa |
| NCBI accession no. | BC017276   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P19320 |
| Biochem/physiol Actions: | Vascular cell adhesion molecule 1 (VCAM1) plays a role in the adhesion of monocytes, lymphocytes, basophils and eosinophils to the vascular endothelium. It also functions in signal transduction and adhesion of leukocytes to endothelial cells. The protein has been associated with atherosclerosis and rheumatoid arthritis. VCAM1 is upregulated in idiopathic pulmonary fibrosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vascular cell adhesion molecule 1 (VCAM1) is activated by transforming growth factor β-1 (TGF-β1) and is part of the immunoglobulin superfamily. The protein is expressed in blood vessels. VCAM1 possesses many extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic domain. The gene encoding it is localized on human chromosome 1p21.2. |
| Immunogen: | VCAM1 (AAH17276, 1 a.a. ~ 739 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPGKMVVILGASNILWIMFAASQAF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |