Monoclonal Anti-RPLP0 antibody produced in mouse
SIGMA/SAB1402899 - clone 1B4, purified immunoglobulin, buffered aqueous solution
Synonym: L10E; MGC111226; MGC88175; P0; PRLP0; RPP0
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to RPLP0 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting RPLP0 monoclonal antibody, clone 1B4. Western Blot analysis of RPLP0 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (60.61 kDa).
ELISA Detection limit for recombinant GST tagged RPLP0 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~60.98 kDa |
| NCBI accession no. | BC015173   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P05388 |
| General description: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq) |
| Immunogen: | RPLP0 (AAH15173, 1 a.a. ~ 317 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPREDRATWKSNYFLKIIQLLDDYP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |