Monoclonal Anti-NME2 antibody produced in mouse
SIGMA/SAB1400187 - clone 1D3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC111212; Anti-NDPKB; Anti-NM23B; Anti-NM23H2; Anti-puf
MDL Number: MFCD01865221
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NME2 monoclonal antibody, clone 1D3. Western Blot analysis of NME2 expression in PC-12.
Western Blotting NME2 monoclonal antibody, clone 1D3. Western Blot analysis of NME2 expression in NIH/3T3.
Western Blotting NME2 monoclonal antibody, clone 1D3 Western Blot analysis of NME2 expression in HeLa.
Western Blotting NME2 monoclonal antibody, clone 1D3. Western Blot analysis of NME2 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (36.96 kDa).
ELISA Detection limit for recombinant GST tagged NME2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3κ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P22392  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ′A′ (encoded by NME1) and ′B′ (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. (provided by RefSeq) |
| Immunogen: | NME2 (NP_002503, 51 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence HYIDLKDRPFFPGLVKYMNSGPVVA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |