Monoclonal Anti-SMAD3 antibody produced in mouse
SIGMA/SAB1400160 - clone 7F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZP586N0721; Anti-DKFZp686J10186; Anti-HSPC193; Anti-HsT17436; Anti-JV152; Anti-MADH3; Anti-MGC60396; Anti-Smad 3
MDL Number: MFCD02097405
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting SMAD3 monoclonal antibody, clone 7F3. Western Blot analysis of SMAD3 expression in HeLa.
Western Blotting SMAD3 monoclonal antibody, clone 7F3. Western Blot analysis of SMAD3 expression in PC-12.
Western Blotting Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody, clone 7F3. Lanes Lane 1: SMAD3 transfected lysate (48 kDa). Lane 2: Non-transfected lysate.
Western Blotting SMAD3 monoclonal antibody, clone 7F3. Western Blot analysis of SMAD3 expression in 293.
Western Blotting SMAD3 monoclonal antibody, clone 7F3. Western Blot analysis of SMAD3 expression in Jurkat.
Western Blotting SMAD3 monoclonal antibody, clone 7F3. Western Blot analysis of SMAD3 expression in human colon.
Western Blotting QC Western Blot detection against Immunogen (36.96 kDa).
ELISA Detection limit for recombinant GST tagged SMAD3 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 7F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P84022  |
| Application: | Monoclonal Anti-SMAD3 antibody produced in mouse has been used in immunohistochemistry (1:200). |
| Biochem/physiol Actions: | Mothers against decapentaplegic homolog 3 (SMAD3) protein possesses tumor-suppressive actions. It is involved in epithelial to mesenchymal transition (EMT). SMAD3 also possesses tumor-promoting actions of transforming growth factor-beta (TGF-β). The N-terminal domain Mad homology 1 (MH1) of SMAD3 plays a role in DNA binding. The SMAD3 gene mutation is linked to an increased incidence of knee osteoarthritis. DNA methylation change in the SMAD3 gene promoter is associated with atopic asthma. SMAD3 protein plays a regulatory role in immune responses. It also modulates fibrosis in the airways. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mothers against decapentaplegic homolog 3 (SMAD3) protein is an important constituent of the transforming growth factor-beta (TGF-β) signaling pathway. Mad homology 1 (MH1) and Mad homology 2 (MH2) are the two functional domains of the SMAD3. The SMAD3 gene is located on human chromosome 15q22.33. |
| Immunogen: | SMAD3 (NP_005893, 120 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VCVNPYHYQRVETPVLPPVLVPRHT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |