SIGMAFAST™ Protease Inhibitor Tablets
SIGMA/S8820 - For General Use
Synonym: Protease Inhibitor Tablets; protease inhibitor
Product Type: Chemical
SigmaFAST Protease Inhibitor Cocktail Tablets for General Use: Cat. No. S8820
SigmaFAST Protease Inhibitor Cocktail Tablet, EDTA-free. Protease inhibitor cocktail tablets are also available in other formulations.
| biological source | bovine lung |
| bovine milk | |
| form | tablet |
| Quality Level | 200  |
| solubility | water: 1 tablet/100mL, clear to hazy, colorless |
| storage temp. | 2-8°C |
| Application: | SIGMAFAST Protease Inhibitor Tablets has been used: • to wash raw semen for membrane protein analysis • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA) |
| Application: | These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis. |
| Biochem/physiol Actions: | Inhibits serine, cysteine, aspartic, and metalloproteases. |
| Features and Benefits: |
|
| General description: | SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract. |
| General description: | The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | AEBSF, 2 mM Aprotinin, 0.3 μM Bestatin, 130 μM EDTA, 1 mM E-64, 14 μM Leupeptin, 1 μM |
| Other Notes: | For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. |
| Physical form: | Tablet that is water soluble. |
| Preparation Note: | One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | C |
| Risk Statements | 34 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |