Superoxide Dismutase from bovine liver
SIGMA/S8409 - ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)
Synonym: SOD; Superoxide: superoxide oxidoreductase
CAS Number: 9054-89-1
EC Number: 232-943-0
MDL Number: MFCD00132404
Product Type: Chemical
Multiple enzymatic scavengers are utilized by the cell to limit damage from reactive oxygen species. These scavengers include members of the superoxide dismutase (SOD) family, catalase, and glutathione peroxidase.
| form | ammonium sulfate suspension |
| mol wt | 32.5 kDa |
| Quality Level | 200  |
| specific activity | 2,000-6,000 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| UniProt accession no. | P00442  |
| Analysis Note: | For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969). |
| Application: | Superoxide Dismutase from bovine liver has been used:
|
| Biochem/physiol Actions: | Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice. |
| General description: | Research area: Cell Signaling Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide. |
| Other Notes: | One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min. |
| Packaging: | 3000, 15000 units in glass bottle |
| Physical form: | Suspension in 3.8 M (NH4)2SO4, pH 7.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2,000-6,000 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.15.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |