Anti-Sonic Hedgehog antibody, Mouse monoclonal
SIGMA/S8321 - clone SH154, purified from hybridoma cell culture
Synonym: Anti-Shh
MDL Number: MFCD06798717
Product Type: Chemical
Immunoblotting SHH recombinant protein extract (Cat. No. S0191) was separated on SDS-PAGE, blotted with Monoclonal Anti-Sonic Hedgehog (SHH) Clone: SH154 (Cat. No. S8321) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 6 μg/mL antibody 2. 3 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SH154, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections |
| indirect ELISA: 2 μg/mL | |
| western blot: 2 μg/mL using recombinant mouse SHH peptide | |
| UniProt accession no. | Q15465  |
| Application: | Anti-Sonic Hedgehog antibody may be used for immunoblotting at a working concentration of ~2 μg/ml. A working antibody concentration of ~2 μg/ml is recommended for ELISA. For immunohistochemistry a dilution of 1:500 may be used. |
| Application: | Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody may be used in ELISA, immunoblotting, and immunohistochemistry. It is used as a probe to determine the presence and roles of Sonic Hedgehog in processes such as patterning during embryogenesis. |
| Biochem/physiol Actions: | Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody recognizes human, rat, and mouse Shh. |
| Biochem/physiol Actions: | Sonic Hedgehog (Shh) is an important cell signaling molecule expressed during embryonic development. Shh is involved in patterning of the developing embryonic systems such as the nervous system, somites, and limbs. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. Shh protein is expressed in key embryonic tissues such as the Hensen’s node, zone of polarizing activity in the posterior limb bud, notochord, and floor plate of the neural tube. Downstream targets of Shh include the transcription factors Gli3, responsible for Greigs polycephalosyndactyly in humans, and Hoxd13, responsible for polysyndactyly. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas Anti-Sonic Hedgehog antibody recognizes human, rat and mouse Shh. |
| Physical form: | Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |