Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in mouse
SIGMA/S8316 - ~2 mg/mL, clone 44.1, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD04040947
Product Type: Chemical
Immunofluorescence Hela cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 20 μg/mL Monoclonal Anti-SUV39H1 Histone Methyltransferase Clone: 44.1 (Cat. No. S8316).The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate (Cat. No. 62197).
Western Blotting HeLa nuclear extract were separated on SDS-PAGE and probed with Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in Mouse, Clone: 44.1 (Cat. No. S8316). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 1:10,000 ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 44.1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using extract of HeLa nuclear cells | |
| UniProt accession no. | O43463  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody is suitable for use in ELISA, immunoblotting (~47 kDa), immunoprecipitation, and immunoncytochemistry applications. For immunoblotting, a working concentration of 2-4 mg/mL is recommended using an extract of HeLa nuclear cells. |
| Biochem/physiol Actions: | Combined disruption of both mouse SUV39H1 and 2 causes chromosomal instability and increased risk of tumorigenesis, as well as complete spermatogenic failure that is caused by non-homologous chromosome associations. |
| Biochem/physiol Actions: | Monoclonal Anti-SUV39H1 Histone Methyltransferase recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase. |
| Biochem/physiol Actions: | Post -translational modification of histones, such as phosphorylation, acetylation, and methylation, are crucial for chromatin remodeling. SUV39H1 and Suv39h1 are the human and mouse homologs of Drosophila Su(var)3-9 protein, respectively. These proteins selectively methylate histone H3 at lysine 9 creating a high-affinity binding site for the HP1 proteins (heterochromatin protein 1). HP1 proteins are known to interact with transcription suppressor proteins, suggesting that the interactions with SUV39H1 and methylated histone H3 mediate a silence effect on the transcription of target genes. Over-expression of SUV39H1 in HeLa cells causes a redistribution of endogenous HP1 proteins and growth retardation, suggesting that SUV39H1-mediated modulation of heterochromatin can impair cell cycle progression. The overall identity between the human and mouse SUV39H1 amino acid sequences is 95%, both lacking an N-terminal 155 amino acid stretch from Drosophila Su(var)3-9. As a consequence, cross-species amino acid identity reaches 42% between the fly and the two mammalian proteins. The SUV39H1 protein consists of three regions: a SET domain, a 110 amino acid domain containing several cysteine conserved residues, and a chromo domain. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-SUV39H1 Histone Methyltransferase (mouse IgG1 isotype) is derived from the 44.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with the recombinant fusion protein MBP-SUV39H1. |
| Immunogen: | recombinant fusion protein MBP-SUV39H1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |