Anti-Staphylococcal Enterotoxin A antibody produced in rabbit
SIGMA/S7656 - whole antiserum
Synonym: Anti-Ent A
MDL Number: MFCD00082016
Product Type: Chemical
ELISA Anti-Staphylococcal Enterotoxin A antibody produced in Rabbit (Cat. No. S7656), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919), was serially diluted to detect Staphylococcal Enterotoxin A by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Staphylococcus aureus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:20,000 using using purified Staphyloccal Enterotoxin A immobilized on nitrocellulose membranes (protein concentation: 50 ng/dot). |
| indirect ELISA: 1:20,000 using using Staphylococcal enterotoxin A |
| Application: | Anti-Staphylococcal Enterotoxin A antibody has been used in western blotting, and enterotoxin A detection by ELISA (enzyme-linked immunosorbent assay). |
| Application: | Anti-Staphylococcal Enterotoxin A antibody produced in rabbit was used as a control to SEA-conjugated MUSE11 antibody produced in xenografted SCID mice. |
| Biochem/physiol Actions: | Staphylococcal enterotoxin A (SEA) from Staphylococcal aureus is leading agent that causes of food poisoning. A concentration of as little as 0.5 mg/ml is sufficient to result in nausea, vomiting, diarrhea and cramps. It stimulates the cell proliferation of peripheral lymphocytes, induces the production of interferons and is important for gut immunity against S. aureus infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The antibody is positive against Staphylococcal enterotoxin A, negative versus Staphylococcal enterotoxin B, Cholera toxin, and Pseudomonas exotoxin A (protein concentration: 50-500 ng/dot). The antibody has not been tested for neutralization potency against active Staphylococcal enterotoxin A. |
| Immunogen: | enterotoxin A from Staphylococcus aureus. |
| Packaging: | 1 mL in glass bottle |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |