SYBR® Green JumpStart™ Taq ReadyMix™
SIGMA/S4438 - for quantitative PCR, MgCI2 in buffer
Synonym: Hot start PCR master mix; sybr green PCR master mix; sybr green qPCR
MDL Number: MFCD03458666
Product Type: Chemical
Ct values for the lambda amplicon using SYBR Green JumpStart Taq ReadyMix. Quantitative PCR (qPCR) was performed on pBac-2cp. Initial template copy number was 106 and was diluted 10-fold in subsequent wells. Threshold cycles (Ct) were determined using the ABI PRISM 7700 Sequence Detection software, and were found to be 15.304 (106), 18.848 (105), 22.883 (104), 26.208 (103), 29.821 (102), 33.398 (101), 37.038 (100), and 40 (0).
Representative kit contents
| application(s) | agriculture |
| compatibility | ABI 7000 |
| ABI 7300 | |
| ABI 7500 | |
| ABI 7500 Fast | |
| ABI 7700 | |
| ABI 7900 | |
| ABI 7900 HT | |
| ABI 7900 HT Fast | |
| ABI StepOne | |
| ABI StepOnePlus | |
| ABI ViiA 7 | |
| Bio-Rad CFX384 | |
| Bio-Rad CFX96 | |
| Bio-Rad iCycler iQ | |
| Bio-Rad iQ 5 | |
| Bio-Rad MiniOpticon | |
| Bio-Rad MJ Chromo4 | |
| Bio-Rad MJ Opticon | |
| Bio-Rad MJ Opticon 2 | |
| Bio-Rad MyiQ | |
| Cepheid SmartCycler | |
| Eppendorf® Mastercycler ep realplex | |
| Eppendorf® Mastercycler ep realplex2 S | |
| Illumina Eco qPCR | |
| Qiagen Corbett Rotor-Gene 3000 | |
| Qiagen Corbett Rotor-Gene 6000 | |
| Qiagen Corbett Rotor-Gene Q | |
| Roche LightCycler 480 | |
| Strategene Mx3000P | |
| Strategene Mx3005P | |
| Strategene Mx4000 | |
| for use with ABI 5700 | |
| concentration | 1.25 units/reaction (50 μL reaction volume) |
| detection method | SYBR® Green |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for (quantitative PCR) |
| technique(s) | qPCR: suitable |
| usage | sufficient for 100 reactions |
| sufficient for 20 reactions | |
| sufficient for 500 reactions |
| Application: | SYBR® Green JumpStart™ Taq ReadyMix™ has been used: • in quantitative PCR (qPCR) to amplify a single area of conservation from all variants of myelin basic protein (mbp), SRY-Box 10 (sox10), and β-actin genes • in quantitative real-time PCR to detect the accumulation of PCR product at every cycle of amplification • in routine qPCR amplifications of DNA template and cDNA template • for amplification of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR) |
| Biochem/physiol Actions: | SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye. SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq. |
| Features and Benefits: | • Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield • SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments • This master mix allows consistency from one reaction to the next • Designed to minimize contamination • Reduced primer dimers • Reduced set-up time as compared to manual or wax HotStart® methods • Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications |
| General description: | SYBR® Green JumpStart™ Taq ReadyMix™ combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values. |
| General description: | SYBR® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | HOTSTART is a registered trademark of Molecular BioProducts, Inc. |
| Legal Information: | JumpStart is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Packaging: | Default reaction volume is 50 μL 20RXN is packaged as 1 X 500 μL 100RXN is packaged as 1 X 2.5 mL 400RXN is packaged as 1 X 10 mL 500RXN is packaged as 1 X 12.5 mL |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |