Human Umbilical Vein Endothelial Cells: HUVEC: Pre-Screened for Angiogenesis & VEGF signaling, neonatal
SIGMA/S200-05N
Synonym: HUVEC cells
Product Type: Product-on-demand
Cell Applications : (A) Human Umbilical Vein Endothelial Cells, HUVEC; (B) HUVEC immunolabeled with VEGFR2 antibodies (green); (C) HUVEC stained with DiI-Ac-LDL (red), the acetylated apoprotein specifically recognized and endocytosed by endothelial cells; (D) HUVEC form vessel-like CD31/PECAM positive (green) structures when cultured with Human Dermal Fibroblasts in the presence of VEGF. Nuclei are visualized with PI (B red) or DAPI (D, blue).
Cell Applications : Top panel: activation of VEGFR-2 signaling in HAOEC, HUVEC and HMVEC by 100 ng/mL VEGF for 0, 5 or 15 minutes. VEGFR-2 autophosphorylation was probed using a phospho-VEGFR-2 (Y1175) specific antibody, Akt activation was detected using a phospho-Akt (S473) specific antibody, and MAP kinase activation was detected using a phospho-p44/42 MAP kinase (T202/Y204) antibody. Bottom panel: pre-screened HUVEC and HMVEC express key endothelial markers Tie2, eNOS, Axl and Etk/Bmx.
| biological source | human blood vessels |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Endothelial |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | arthritis; diabetes; cardiovascular diseases; cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | angiogenesis, cell signaling pathways, migration, invasion, growth factors, tube formation, vascular homeostasis, modulation of blood vessel tone, regulate cell grwoth and ECM deposition, vasculoregulation |
| Biochem/physiol Actions: | Blood Vessels |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Endothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development. Cell Applications, Inc. offers the widest variety of endothelial cells from different vascular beds. Matched smooth muscle cells obtained from the same arteries are available as well. The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx. VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK. Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells Prescreened Endothelial Cells express the following important biomarkers: • Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes; • eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function; • Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation. • Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival. |
| Other Notes: | Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO |
| Preparation Note: | • Primary culture, >500,000 cells in Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 16 doublings |
| Preparation Note: | Please refer to the HUVEC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |