Sulfatase from Aerobacter aerogenes
SIGMA/S1629 - Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Synonym: Aryl-sulfatase; Aryl-sulfate sulfohydrolase; Phenolsulfatase
CAS Number: 9016-17-5
MDL Number: MFCD00132399
Product Type: Chemical
Mechanism of Sulfatase from Aerobacter aerogenes (Cat. No. S1629) Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates.
| concentration | 10-20 units/mL |
| foreign activity | β-Glucuronidase ≤10 U/mL |
| form | buffered aqueous glycerol solution |
| mol wt | ~41 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | 2-5 units/mg protein (biuret) |
| storage temp. | −20°C |
| type | Type VI |
| Application: | Sulfatase from Aerobacter aerogenes has been used: • as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses • in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1 • to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides |
| Biochem/physiol Actions: | Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture. |
| General description: | Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C. |
| Physical form: | Solution in 50% glycerol containing 0.01 M Tris, pH 7.5. |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2-5 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.6.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |