MISSION® siRNA Transfection Reagent
SIGMA/S1452
Synonym: MISSION® siRNA Transfection Reagent
Product Type: Chemical
| form | aqueous solution |
| product line | MISSION® |
| Quality Level | 200 ![]() |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| General description: | The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression. Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics. |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection. |
| Symbol | GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Risk Statements | 10 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 120.2 °F |
| Flash Point(C) | 49 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106502 |


