Imprint® RNA Immunoprecipitation Kit
SIGMA/RIP - High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,
suitable for use with mRNA and microRNA
Synonym: Magnetic Bead RNA Immunoprecipitation; RNA Immunopreciptation; mRNA Immunopreciptation; microRNA Immunoprecipitation
Product Type: Chemical
Workflow for RIP
RIP Lysate prepared from HeLa cells (0.75 × 106 cell equivalents per IP) were immunoprecipitated using 1 μg of either a normal Rabbit IgG, or Anti-SNRP70 antibody and the Imprint RNA Immunoprecipitation Kit. Immunoprecipitation of SNRP70-associated RNA was validated by qPCR using Control Primers, U1 snRNA.
RIP Lysate prepared from HeLa cells (0.75 × 106 cell equivalents per IP) were immunoprecipitated using 1 μg of either a normal mouse IgG, or Anti-PABPC1 antibody along with Anti-Mouse antibody produced in Rabbit and the Imprint RNA Immunoprecipitation Kit. Immunoprecipitation of PABP-associated RNA was validated by qPCR using Control Primers, Actin B
RIP Lysate prepared from HeLa cells (2.0 × 106 cell equivalents per IP) were immunoprecipitated using 1 μg of either a normal Rabbit IgG, or Anti-HUR antibody and the Imprint RNA Immunoprecipitation Kit. Immunoprecipitation of HUR-associated RNA was validated by qPCR using Control Primers, Actin B
RIP Lysate prepared from HeLa cells (0.5 × 106 or 1.7 × 106 cell equivalents per IP) were immunoprecipated using 2.5 μg of either a normal Rabbit IgG or Anti-HUR antibody and the Imprint RNA Immunoprecipitation Kit. Immunoprecipitation of HUR-target and non-target associated RNA was validated by qPCR using Control Primers Actin B and JUN
| Quality Level | 200  |
| Application: | Imprint® RNA Immunoprecipitation Kit has been used for: • Suitable for downstream applications • Individual target characterization to genome-wide profiling techniques • Characterization of signal transduction pathways • Verification of ChIp-chIP and ChIP-seq data |
| Features and Benefits: | • Xtra Protein A magnetic beads with higher binding capacity • Step-by-step protocols for cell lysis and immunoprecipitation • RNAse inhibitors and RNAse-free reagents to maintain RNA integrity • Negative control and antibody included |
| General description: | Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP. Learn more about this product and view application data . |
| General description: | RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR. |
| Legal Information: | Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol |   GHS02,GHS07 |
| Signal word | Warning |
| Hazard statements | H226 - H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| Risk Statements | 10 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 100.4 °F |
| Flash Point(C) | 38 °C |
| UNSPSC | 41116158 |
| Components | IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder; IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder; Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution; Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution; Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL |