Thrombin CleanCleave™ Kit
SIGMA/RECOMT
Synonym: Thrombin Cleavage Kit
Product Type: Chemical
a. P4 and P3 are hydrophobic, P1′ and P2′ are nonacidic residues, and Arg (or Lys)•P1′ is the scissile bond.
b. P2 or P1′ is Gly and Arg (or Lys)•P1′ is the scissile bond.
b. P2 or P1′ is Gly and Arg (or Lys)•P1′ is the scissile bond.
| form | suspension |
| mol wt | 37 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | protein purification: suitable |
| Application: | For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems. |
| Features and Benefits: | • Fast, efficient cleavage in as little as 2 hours • Thrombin is covalently bound to agarose for easy removal. • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths. • Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA • Thrombin-agarose is reusable. |
| General description: | The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins |
| Legal Information: | CleanCleave is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Preparation Note: | 200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Thrombin Cleavage Buffer, 10× 10 mL |