Human BDNF ELISA Kit
SIGMA/RAB0026 - for serum, plasma, cell culture supernatant and urine
Synonym: BDNF; Brain-derived neurotrophic factor
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human BDNF ELISA Kit, Cat. No. RAB0026. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human BDNF ELISA Kit, Cat. No. RAB0026, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 80 pg/mL standard curve range: 0.066-16 ng/mL |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) sample type urine |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats. It has also been used to determine the plasma BDNF levels in GD1 (Gaucher’s disease type 1) patients and control population. |
| Application: | Human BDNF ELISA Kit has been used: • to measure the brain-derived neurotrophic factor (BDNF) protein expression • to determine BDNF level in the slice culture medium and the extracts of organotypic brain slice cultures (OBSCs) • to determine BDNF level in plasma |
| Biochem/physiol Actions: | Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus. |
| General description: | The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. |
| General description: | The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human BDNF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |