Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal
SIGMA/R3155 - clone RabT-50, purified from hybridoma cell culture
Synonym: Mouse Anti-Rabbit IgG
Product Type: Chemical
Western Blotting Whole extract of NIH-3T3 cells were separated on SDS-PAGE and probed with 1:250 Anti-Actin (20-33) antibody produced in Rabbit (Cat. No. A5060). The antibody was developed using Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in Mouse, Clone: RabT-50 (Cat. No. R3155). Lanes 1. 1:20,000 antibody 2. 1:10,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RabT-50, monoclonal |
| concentration | 2.5-5.0 mg/mL (antibody) |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human, horse, bovine, cat, rat, chicken, pig, goat, monkey |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody | |
| western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody. |
| Application: | Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • western blotting |
| Biochem/physiol Actions: | Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP). |
| General description: | Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies. |
| Immunogen: | Enriched fraction of rabbit IgG light chain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |