RPMI-1640 Medium
SIGMA/R0883 - With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: Roswell Park Memorial Institute 1640 medium
MDL Number: MFCD00217820
Product Type: Chemical
| components | sodium pyruvate: no phenol red: yes L-glutamine: no NaHCO3: yes HEPES: no |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | RPMI-1640 Medium has been used: • to culture PC-3 prostate cell line and Jurkat cells • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line • to cultivate circulating tumor cells (CTCs) |
| General description: | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes. |
| Preparation Note: | Supplement with 0.3 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |