Protease and Phosphatase Inhibitor Cocktail
SIGMA/PPC1010
Synonym: Protease and Phosphatase Inhibitor Mix; Protease and phosphatase inhibitor
Product Type: Chemical
Bowman–Birk protease inhibitor. Proteins of this family interact with the enzymes they inhibit via an exposed surface loop that adopts the canonical proteinase inhibitory conformation.
| form | DMSO solution |
| packaging | pkg of 1 ml, 5 ml × |
| Quality Level | 200  |
| shipped in | wet ice |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer: • for cellular protein extraction • to lyse purified CD4+ T cells • to prepare protein extracts from placental tissues and cells |
| Application: | Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers. |
| General description: | Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. |
| Preparation Note: | The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer. |
| WGK Germany | WGK 2 |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |