Goat anti-RNA Polymerase II Antibody, Affinity Purified
SIGMA/PLA0292 - Powered by Bethyl Laboratories, Inc.
Synonym: 220kDa; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; POLR2; POLRA; RNA polymerase II 220 kd subunit; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; polymerase (RNA) II (DNA directed) polypeptide A
Product Type: Product-on-demand
Western Blotting Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Human RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg), and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (Cat. No. PLA0292) used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blotting Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (Cat. No. PLA0292) used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II antibody Cat. No. PLA0292 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated RNA Polymerase II, PLA0292 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified Goat Anti-RNA Polymerase II antibody Cat. No. PLA0292 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated RNA Polymerase II, PLA0292 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_000928.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2- 10 μg/mg |
| western blot: 1:1,000- 1:5,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0292 maps to a region within the C-terminal repeat of human RNA Polymerase II using the reference sequence NP_000928.1 (GeneID 5430). |
| Other Notes: | The RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II core complex. RNA polymerase II holoenzyme functions to transcribe DNA into messenger RNA in eukaryotes. RPB1 forms the DNA binding domain of the polymerase that forms a cleft that grabs the incoming DNA template to translocate the holoenzyme across the RNA-DNA hybrid. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |