PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
SIGMA/PKH67GL - Distributed for Phanos Technologies
Synonym: Green PKH membrane labeling kit
Product Type: Chemical
Internalization kinetics of PKH67 in L929 cells. (Photo courtesy of Cecile Rousselle and Dr. Xavier Ronot, Universite Joseph Fourier, Grenoble, France)
PKH67 spectra
| detection method | fluorometric |
| fluorescence | λex 490 nm; λem 502 nm (PKH67 dye) |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| shipped in | ambient |
| storage condition | protect from light |
| storage temp. | room temp |
| Application: | PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used: • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition. • To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells. • In fluorescence imaging. |
| Application: | This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length. |
| Legal Information: | CellVue is a registered trademark of Phanos Technologies |
| Other Notes: | For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here . |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 |
| Hazard Codes | F |
| Risk Statements | 11 |
| Safety Statements | 7-16 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 57.2 °F - closed cup |
| Flash Point(C) | 14 °C - closed cup |
| Storage Temp. | room temp |
| UNSPSC | 12352207 |
| Components | Diluent C 6 x 10 mL; PKH67 Cell Linker in ethanol .5 mL |