Protease Inhibitor Cocktail
SIGMA/P9599 - for plant cell and tissue extracts, DMSO solution
Synonym: Protease Inhibitor Mix
MDL Number: MFCD00677817
Product Type: Chemical
The five graphs above present endogenous protease activities in plant tissues and their inhibition by Sigma-Aldrich plant cell protease inhibitor cocktail.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9599-1ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9599-5ML
| form | DMSO solution |
| Quality Level | 300  |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | This product has been specifically tested on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat, and tobacco. The recommended quantity is one mL of solution for the inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells. |
| Biochem/physiol Actions: | Inhibits serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases |
| Biochem/physiol Actions: | This mixture contains individual components, including AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64. Each component has specific inhibitory properties. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases. 1,10-Phenanthroline acts against metalloproteases. |
| Disclaimer: | The cocktail should be stored at -20°C, where it will retain stability for two years. |
| Features and Benefits: | Contains a broad specificity of inhibitory properties including serine, cysteine, aspartic and metalloproteases, and aminopeptidases Optimized and tested for use with plant tissue and cell extracts Specifically tested on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat, and tobacco One mL of solution is recommended for the inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells |
| General description: | Our Protease Inhibitor Cocktail is a mixture of individual components optimized and tested for use with plant tissue and cell extracts. The cocktail contains a broad specificity of inhibitory properties including serine, cysteine, aspartic and metalloproteases, and aminopeptidases. The individual components include AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64. |
| Other Notes: | AEBSF Bestatin E-64 Leupeptin Pepstatin A 1,10-Phenanthroline |
| Packaging: | 1, 5 mL in glass bottle |
| Preparation Note: | One mL is recommended for the inhibition of proteases extracted from 30 g of plant tissue in a total volume of 100 ml. |
| Preparation Note: | This product is supplied as a clear, faint pink solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells. Extracts of plant seedlings from pea, bean, wheat, tobacco, and Arabidopsis have been tested. The roots of these plants have also been successfully tested. |
| Risk Statements | 36/38 |
| Safety Statements | 24-26 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 185.0 °F - closed cup |
| Flash Point(C) | 85 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |